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KMID : 1024420100140010021
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Food Engineering Progress
2010 Volume.14 No. 1 p.21 ~ p.26
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Glass Slide-based Immunosensing for C-Reactive Protein Using Quantum Dot-Antibody Conjugate
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Kim Nam-Soo
Oh Sun-Mi
Kim Chong-Tai
Cho Yong-Jin
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Abstract
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-Reactive protein (CRP), which is an 118 kDa pentameric protein, was secreted by the liver is an important biomarker for coronary disease, hypertension and inflammation. In this study, a method for CRP detection exploiting quantum dot (Qdot)-antibody conjugate was developed according to an indirect-competitive immunosensing protocol. For this purpose, a streptavidin-bound Qdot605 was linked with a separately prepared biotinylated monoclonal anti-rat CRP antibody to produce a Qdot-antibody conjugate. The immunosensing was performed at 0.1 and 20 nM of the coating antigen and conjugate, respectively. The current method was found very sensitive in CRP detection, judging from the concentration-dependent fluorescence emission.
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KEYWORD
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glass slide, immunosensing, C-reactive protein, quantum dot-antibody conjugate
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